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loading control b actin  (Proteintech)


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    Structured Review

    Proteintech loading control b actin
    Loading Control B Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 8335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+actin/beta+Actin+Monoclonal+antibody/pm42854903-131-60-64
    Average 97 stars, based on 8335 article reviews
    loading control b actin - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Analysis:

    Article Title: H4K5 lactylation exacerbates acute myocarditis by driving a positive feedback loop between inflammation and metabolic dysfunction.
    Article Snippet: Equal amounts of protein were then resolved by 8–12% SDS-PAGE using Mini Protean precast gels (Bio-Rad Laboratories). .. Protein homogenates were subjected to analysis using antibodies against PKM2 (4053, CST), HK2 (3582, CST), LDHA (2867, CST), Panla (PTM-1425RM, PTM Biolabs), H4K5la (PTM-11407RM, PTM Biolabs), H4K8la (PTM-1415RM, PTMBiolabs), H4K12la (PTM-1411RM, PTMBiolabs), H4 (A19815, Abclonal), iNOS (ab283655, Abcam), Arg1 (ab259271,Abcam), TNFR1 (3736,CST), T-P65 (8242,CST), P-P65 (3033,CST), TNF-a (11948,CST), Rap1 (A7981, Abclonal), Bcl-2 (ab182858, Abcam), Bax frontiersin.org (ab32503, Abcam), H4K5ac (PTM-163, PTM Biolabs), P300 (ab10485, Abcam) and b-actin (HRP-66009, Proteintech). ..

    other:

    Article Title: Pan-cancer analysis of NTRK2 (TRKB) and the anticancer effect of its inhibitor Lucitanib in glioma.
    Article Snippet: Neurotrophic tyrosine receptor kinase 2 (NTRK2/TRKB) demonstrates oncogenic roles across cancers, with notable significance in gliomas where its overexpression is linked to aggressive clinical phenotypes.. Lucitanib (AL3810), a multi-target tyrosine kinase inhibitor, shows unexplored potential for treating NTRK2-driven gliomas.. This study employed an integrated approach combining pan-cancer analysis, computational drug screening, and experimental validation to systematically evaluate the oncogenic function of NTRK2 and the therapeutic efficacy of Lucitanib.

    Incubation:

    Article Title: Targeting NLRP3 Inflammasome and Metabolic Dysregulation by Electroacupuncture: A Novel Therapeutic Strategy for Methamphetamine Withdrawal-Induced Depression.
    Article Snippet: Equal amounts of protein (20 mg per sample) were separated on 10% SDS-PAGE gels (M00654, Gene Script, China) and transferred to PVDF membranes (3010040001, Millipore, USA). .. Membranes were blocked with 5% non-fat milk (P0216, Beyotime, China) for 1 hour at room temperature and incubated overnight at 4 �C with primary antibodies against Occludin (ab216327, 1:1000, Abcam, UK), Claudin-5 (AF5216, 1:500, Affinity Biosciences), ZO-1 (61-7300, 1 mg/mL, Invitrogen, USA), NLRP3 (30109-1-AP, 1:1000, Proteintech, China), ASC (DF6304, 1:500, Affinity Biosciences), IL1b (ab315084, 1:1000, Abcam, UK), and b-Actin (20536-1-AP, 1:5000, Proteintech, China). .. The next day, membranes were washed and incubated for 1 hour with an HRP-conjugated goat anti-rabbit IgG secondary antibody (SA00001-2, 0.2 mg/mL, Proteintech, China).

    Article Title: Thioredoxin-1 Inhibits Golgi Stress Induced by Methyl-4-Phenyl-1,2,3, 6-Tetrahydropyridine.
    Article Snippet: Aims: Parkinson’s disease (PD) is a common neurodegenerative disease characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc) and the aggregation of alphasynuclein (a-syn) in Lewy bodies.. Emerging studies find that disruption of the Golgi structure and Golgi stress are involved in PD.. Thioredoxin-1 (Trx-1) is a redox regulatory protein that protects DA neurons frommethyl-4phenyl-1,2,3, 6-tetrahydropyridine (MPTP) damage.

    Western Blot:

    Article Title: Adhesion molecule with Ig-like domain 1 regulates stability of carotid plaque via TGFβ/Smad signaling pathway by interaction with TGFRII.
    Article Snippet: Carotid atherosclerosis is a significant risk factor for cardiovascular and cerebrovascular diseases.. Maintaining plaque stability can prevent plaque rupture and thrombus formation, slow disease progression, and is critically important for preventing cerebrovascular events (such as stroke, transient ischemic attack (TIA), and similar events).. Mechanisms influencing plaque stability are still unclear.

    Article Title: Thioredoxin-1 Inhibits Golgi Stress Induced by Methyl-4-Phenyl-1,2,3, 6-Tetrahydropyridine.
    Article Snippet: Aims: Parkinson’s disease (PD) is a common neurodegenerative disease characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc) and the aggregation of alphasynuclein (a-syn) in Lewy bodies.. Emerging studies find that disruption of the Golgi structure and Golgi stress are involved in PD.. Thioredoxin-1 (Trx-1) is a redox regulatory protein that protects DA neurons frommethyl-4phenyl-1,2,3, 6-tetrahydropyridine (MPTP) damage.

    Immunohistochemistry:

    Article Title: Adhesion molecule with Ig-like domain 1 regulates stability of carotid plaque via TGFβ/Smad signaling pathway by interaction with TGFRII.
    Article Snippet: Carotid atherosclerosis is a significant risk factor for cardiovascular and cerebrovascular diseases.. Maintaining plaque stability can prevent plaque rupture and thrombus formation, slow disease progression, and is critically important for preventing cerebrovascular events (such as stroke, transient ischemic attack (TIA), and similar events).. Mechanisms influencing plaque stability are still unclear.

    Activity Assay:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    Cell Counting:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    Food & Beverages:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    ROS Assay:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    Membrane:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    Fluorescence:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..

    Cell Culture:

    Article Title: Prebiotic augmented nanozyme-armored probiotics reprogramme the gut microenvironment for synchronous treatment of colitis and sepsis via antioxidant defense.
    Article Snippet: .. The chemical reagents used in this study are summarized as below: cerium nitrate hexahydrate (CeN3O9 6H2O, Aladdin), ruthenium (III) chloride hydrate (RuCl3 xH2O, Aladdin), Fu brick tea (Qinchuangyuan, Xi’an, China), superoxide dismutase (SOD) activity, catalase (CAT) activity, micro hydroxyl free radical scavenging capacity, and total antioxidant capacity (T-AOC) assay kit (Solarbio), cell counting kit 8 (CCK8, Dojindo), Lactobacillus kefiranofaciens JKSP109 (LK, Health Food Manufacturing Team Strain Center, Northwest University), human colonic epithelial cells (Hcoepic cells SC0615, Shanghai Yuchi Biology Co., Ltd), reactive active species (ROS) assay kit highly sensitive DCFH-DA (Dojindo), cell membrane fluorescent probe DiR (Beijing Fluorescence Biotechnology), Dimethyl sulfoxide (cell culture grade, MP Biomedicals), dextran sulfate sodium salt (DSS, MW: 36–50 kDa, MP Biochemicals), lipopolysaccharide (LPS, Sigma), paraformaldehyde (4%, biosharp), b-actin (20536-1-AP, Proteintech), anticlaudin-1 tight junction protein antibody (13050-1-AP, Proteintech), Hcoepic cell line (American Type Culture Collection), 4′, 6- diamidino-2-phenylindole (DAPI, Beyotime Biotechnology), nearinfrared fluorescent dye sulfo-cyanine5.5 (Cy5.5, Aladdin). ..



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